Article
A complex web of signal-dependent trafficking underlies the triorganellar distribution of P-selectin in neuroendocrine PC12 cells.
The Journal of cell biology - 28 Jun 1999
Blagoveshchenskaya A D, Hewitt E W, Cutler D F
Abstract excerpt
By analyzing the trafficking of HRP-P-selectin chimeras in which the lumenal domain of P-selectin was replaced with horseradish peroxidase, we determined the sequences needed for targeting to synaptic-like microvesicles (SLMV), dense core granules (DCG), and lysosomes in neuroendocrine PC12 cells. Within the cytoplasmic domain of P-selectin, Tyr777 is needed for the appearance of P-selectin in immature and mature...
Topics
- Amino Acid Sequence
- Animals
- Biological Transport
- Biomarkers
- Brefeldin A
- Cell Fractionation
- Cell Membrane
- Cytoplasmic Granules
- Endocytosis
- Endosomes
- Leucine
- Lysosomes
- Molecular Sequence Data
- Mutation
- Organelles