Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of tandem CC-->TT mutations induced by oxygen radicals using mutation-specific PCR.

Mutation research - 1 Jun 1999

Newcomb T G, Allen K J, Tkeshelashvili L, Loeb L A

Abstract excerpt

DNA lesions caused by reactive oxygen species (ROS) are considered to be one of the major contributors to DNA damage and mutagenesis. In this study, we developed a modification of allele-specific PCR to detect CC-->TT mutations caused by oxidative damage. These tandem mutations have been previously demonstrated to be indicative of oxygen damage in the absence of UV-irradiation. Using a CC target site in the rat...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Damage
Share to DNA Polymerase beta
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Free Radicals

Share to HeLa Cells

Share to Hydrogen Peroxide

Share to Plasmids

Share to Reactive Oxygen Species

Source

Identifiers

PMID: 10354498
DOI: 10.1016/S0027-5107(99)00075-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection of tandem CC-->TT mutations induced by oxygen radicals using mutation-specific PCR.PMID 10354498Read ↗

Efficient formation of the tandem thymine glycol/8-oxo-7,8-dihydroguanine lesion in isolated DNA and the mutagenic and cytotoxic properties of the tandem lesions in Escherichia coli cells.Semantic proximity Read ↗

Mutational spectra induced by flavonoid extracts from pepper tree (Schinus terebinthifolius, Raddi) stem bark.Semantic proximity Read ↗

High frequency induction of CC to TT tandem mutations in DNA repair-proficient mammalian cells.Semantic proximity Read ↗

Copper-dependent site-specific mutagenesis by benzoyl peroxide in the supF gene of the mutation reporter plasmid pS189.Semantic proximity Read ↗

A role for Pms2 in the prevention of tandem CC --> TT substitutions induced by ultraviolet radiation and oxidative stress.Semantic proximity Read ↗

Resolution and characterization of polymorphic DNA by SSCP and chemical cleavage methodologies.Semantic proximity Read ↗

Mutations at G:C base pairs predominate after replication of peroxyl radical-damaged pSP189 plasmids in human cells.Semantic proximity Read ↗

Flow cytometric detection of tandem repeat mutations induced by various chemical classes.Semantic proximity Read ↗

Mutagenic fingerprint of ozone in human cells.Semantic proximity Read ↗

Oxidative DNA damage and mutations induced by a polar photosensitizer, Ro19-8022.Semantic proximity Read ↗

An alternate nonisotopic technique of PCR-mediated allele-specific oligonucleotide analysis for the detection of a point mutation.Semantic proximity Read ↗

Site-specific mutagenesis by triple helix-forming oligonucleotides containing a reactive nucleoside analog.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.