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Article

Sequential chemiluminescent detection of target DNAs without stripping and reprobing.

BioTechniques - 1 Apr 1999

Reddy L V, DeSilva R, Handley R S, Schaap A P, Akhavan-Tafti H

Abstract excerpt

We present a simple method for sequential chemiluminescent detections of two different DNA loci on a single Southern blot. First, an enzyme-linked DNA probe for a unique sequence is detected with a horse-radish peroxidase (HRP) substrate followed by the detection of another enzyme-linked DNA probe for a different unique sequence with an alkaline phosphatase (AP) substrate that simultaneously inhibits the...

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PMID: 10343910
DOI: 10.2144/99264st08

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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