Article
Simultaneous amplification and detection of specific DNA sequences.
Bio/technology (Nature Publishing Company) - 1 Apr 1992
Higuchi R, Dollinger G, Walsh P S, Griffith R
Abstract excerpt
We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences can be detected without opening the reaction tube. This enhancement requires the addition of ethidium bromide (EtBr) to a PCR. Since the fluorescence of EtBr increases in the presence of double-stranded (ds) DNA an increase in fluorescence in such a PCR indicates a positive amplification, which can be easily monitored...
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