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Article

Different mechanisms for thermal inactivation of Bacillus subtilis signal peptidase mutants.

The Journal of biological chemistry - 28 May 1999

Bolhuis A, Tjalsma H, Stephenson K, Harwood C R, Venema G, Bron S, van Dijl J M

Abstract excerpt

The type I signal peptidase SipS of Bacillus subtilis is of major importance for the processing of secretory precursor proteins. In the present studies, we have investigated possible mechanisms of thermal inactivation of five temperature-sensitive SipS mutants. The results demonstrate that two of these mutants, L74A and Y81A, are structurally stable but strongly impaired in catalytic activity at 48 degrees C,...

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Source

Identifiers

PMID: 10336490
DOI: 10.1074/jbc.274.22.15865

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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