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Article

A fluorescence investigation of the active site of Pseudomonas aeruginosa exotoxin A.

The Journal of biological chemistry - 28 May 1999

Beattie B K, Merrill A R

Abstract excerpt

Single tryptophan mutant proteins of a catalytically active domain III recombinant protein (PE24) from Pseudomonas aeruginosa exotoxin A were prepared by site-directed mutagenesis. The binding of the dinucleotide substrate, NAD+, to the PE24 active site was studied by exploiting intrinsic tryptophan fluorescence for the wild-type, single Trp, and tryptophan-deficient mutant proteins. Various approaches were used...

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Identifiers

PMID: 10336461
DOI: 10.1074/jbc.274.22.15646

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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