Article
Substitution of a proline for alanine 183 in the hinge region of phosphoglycerate kinase: effects on catalysis, activation by sulfate, and thermal stability.
Journal of protein chemistry - 1 Feb 1990
Bailey J M, Lin L N, Brandts J F, Mas M T
Abstract excerpt
A "hinge-bending" domain movement has been postulated as an important part of the catalytic mechanism of phosphoglycerate kinase (PGK) (Banks et al., 1979). In order to test the role of the flexibility of a putative interdomain hinge in the substrate- and sulfate-induced conformational transition...
Topics
- Alanine
- Calorimetry, Differential Scanning
- Catalysis
- Enzyme Activation
- Hot Temperature
- Mutation
- Oligonucleotides
- Phosphoglycerate Kinase
- Proline
- Protein Conformation
- Protein Denaturation
- Structure-Activity Relationship
- Sulfates
- Yeasts