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Article

Substrate-assisted movement of the catalytic Lys 215 during domain closure: site-directed mutagenesis studies of human 3-phosphoglycerate kinase.

Biochemistry - 27 Dec 2005

Flachner Beáta, Varga Andrea, Szabó Judit, Barna László, Hajdú István, Gyimesi Gergely, Závodszky Péter, Vas Mária

Abstract excerpt

3-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme. It is still unclear how the geometry of the active site is formed during domain closure and how the catalytic residues are brought into the optimal position for the reaction. Comparison of the three-dimensional structures in various open and closed conformations suggests a large (10 A) movement of Lys 215 during domain closure. This change...

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PMID: 16363799
DOI: 10.1021/bi051726g

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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