Article
Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid.
Journal of bacteriology - 1 Mar 1999
Kobashi N, Nishiyama M, Tanokura M
Abstract excerpt
An aspartate kinase-deficient mutant of Thermus thermophilus, AK001, was constructed. The mutant strain did not grow in a minimal medium, suggesting that T. thermophilus contains a single aspartate kinase. Growth of the mutant strain was restored by addition of both threonine and methionine, while addition of lysine had no detectable effect on growth. To further elucidate the lysine biosynthetic pathway in T....
Topics
- 2-Aminoadipic Acid
- Amino Acid Sequence
- Aspartate Kinase
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Diaminopimelic Acid
- Genes, Bacterial
- Genetic Complementation Test
- Hydro-Lyases