Article
Trifluoroleucine resistance and regulation of alpha-isopropyl malate synthase in Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 1 Feb 1999
Cavalieri D, Casalone E, Bendoni B, Fia G, Polsinelli M, Barberio C
Abstract excerpt
Seven spontaneous Saccharomyces cerevisiae mutants that express dominant resistance to 5,5,5-trifluoro-DL-leucine have been characterised at the molecular level. The gene responsible for the resistance was cloned from one of the mutants (FSC2.4). Determination of its nucleotide sequence showed that it was an allele of LEU4 (LEU4-1), the gene that encodes alpha-isopropyl malate synthase I (alpha-IPM synthase I),...
Topics
- 2-Isopropylmalate Synthase
- Alleles
- Amino Acid Sequence
- Blotting, Northern
- Cloning, Molecular
- Coenzyme A
- DNA Mutational Analysis
- Drug Resistance, Microbial
- Gene Deletion
- Genes, Fungal
- Leucine
- Molecular Sequence Data
- Mutation