Article
Detection of neutral and charged mutations in alpha- and beta-human globin chains by capillary zone electrophoresis in isoelectric, acidic buffers.
Journal of chromatography. A - 5 Feb 1999
Saccomani A, Gelfi C, Wajcman H, Righetti P G
Abstract excerpt
A simple and reliable method, for screening for point mutations in alpha- and beta-human globin chains, is reported here, utilizing capillary zone electrophoresis in isoelectric, acidic buffers. A solution of 50 mM iminodiacetic acid (pI 2.23) containing 7 M urea and 0.5% hydroxyethylcellulose (apparent pH 3.2) is used as background electrolyte for fast separation of heme-free, denatured globin (alpha and beta)...
Topics
- Buffers
- Electrophoresis, Capillary
- Erythrocytes
- Globins
- Humans
- Indicators and Reagents
- Isoelectric Focusing
- Mutation