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Article

Expression of human kidney 11beta-hydroxysteroid dehydrogenase (11-HSD2) in bacteria.

Biochemical and biophysical research communications - 24 Feb 1999

Nunez B S, Mune T, White P C

Abstract excerpt

The kidney isozyme of 11beta-hydroxysteroid dehydrogenase (11-HSD2) protects the mineralocorticoid receptor from spurious activation by glucocorticoids. To explore structure-function relationships, human 11-HSD2 cDNA was subcloned into the bacterial expression vector, pET25b. E. coli transformed with wild-type cDNA produced active enzyme that retained biochemical characteristics of the native protein. The...

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Identifiers

PMID: 10049765
DOI: 10.1006/bbrc.1999.0259

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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