Article

Labeling of Nascent RNA in <i>C. elegans</i> Intestine

2025-09-09

Abstract excerpt

Transcriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivo EU-click labeling protocol that enables sensitive microscopy detection of newly transcribed RNA in dissected intestines. Using worms expressing fluorescent nucleolar markers, we show that EU incorporation faithfully reports...

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Literature Corpus work: fdcf2206-091f-5f77-806f-bb60e51df4f2
DOI: 10.1101/2025.09.08.675001

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