Article

Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing

2022-01-28

Abstract excerpt

In order to automate the genome sequencing pipeline in our laboratory, we programmed a dual-arm anthropomorphic robot, the Robotic Biology Institute’s Maholo LabDroid, to perform organic solvent-based genomic DNA extraction from cell lysates. To the best of our knowledge, this is the first time that automation of phenol-chloroform extraction has been reported. We achieved routine extraction of high molecular weigh...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: fd13b0cc-2e85-59c0-9668-59be012ff12e
DOI: 10.1101/2022.01.26.477939

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencingDOI 10.1101/2022.01.26.477939Read

Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencingSemantic proximity Read

Ultra-fast sample-to-sequencing workflow for clinical diagnostics using micropillarsSemantic proximity Read

Rapid, High-Throughput, Cost Effective Whole Genome Sequencing of SARS-CoV-2 Using a Condensed One Hour Library Preparation of the Illumina DNA Prep KitSemantic proximity Read

Haplotype-phased synthetic long reads from short-read sequencingSemantic proximity Read

Use of robotics in high-throughput DNA sequencing.Semantic proximity Read

High molecular weight DNA extraction strategies for long-read sequencing of complex metagenomesSemantic proximity Read

Automation workflow for high-throughput arrayed plasmid DNA preparation and quantificationSemantic proximity Read

An Evaluation of Avian Influenza Virus Whole Genome Sequencing Approaches Using Nanopore TechnologySemantic proximity Read

Optimised use of Oxford Nanopore Flowcells for Hybrid AssembliesSemantic proximity Read

Optimized use of Oxford Nanopore flowcells for hybrid assembliesSemantic proximity Read

Automated Assembly of Programmable RNA-Based SensorsSemantic proximity Read

Rapid, high-throughput, cost-effective whole-genome sequencing of SARS-CoV-2 using a condensed library preparation of the Illumina DNA Prep kitSemantic proximity Read