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Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors

2003-01-01

Abstract excerpt

High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinity-tag based chromatography to overexpress and purify a human therapeutic protein,...

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Literature Corpus work
eb5090ad-bef6-5740-95ca-93fb2b1c36d3
DOI
10.1023/a:1022114427971
Open publication

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Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactorsDOI 10.1023/a:1022114427971
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