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Article

Direct next-generation sequencing of virus-human mixed samples without pretreatment is favorable to recover virus genome

12 Jan 2016

Abstract excerpt

Next-generation sequencing (NGS) enables the recovery of pathogen genomes from clinical samples without the need for culturing. Depletion of host/microbiota components (e.g., ribosomal RNA and poly-A RNA) and whole DNA/cDNA amplification are routine methods to improve recovery results. Using mixtures of human and influenza A virus (H1N1) RNA as a model, we found that background depletion and whole transcriptome...

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PMID: 26754142
DOI: 10.1186/s13062-016-0105-x

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