Article

“In vitro construction and long read sequencing analysis of a 24 kb long artificial DNA sequence encoding the Universal Declaration of the Rights of Man and of the Citizen ”

2023-06-26

Abstract excerpt

In absence of DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. We propose in this report a fully in vitro protocol to generate very long double-stranded DNA m...

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Literature Corpus work: de7dfc13-fe93-5480-92bc-2b3e28205ed6
DOI: 10.1101/2023.06.26.546242

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