Article

Nicotine biosynthesis completed by cryptic activating glucosylation

2025-12-06

Abstract excerpt

Nicotine is a neuroactive alkaloid produced by tobacco ( Nicotiana tabacum ) as a defense against herbivory, and an addictive stimulant that has been used by humans for millennia. Despite its significance, the core steps of its biosynthesis have remained elusive. Here, we demonstrate in vitro reconstruction of nicotine synthase, a four-enzyme stereoselective biocatalytic cascade that forms ( S )-nicotine from n...

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Literature Corpus work: d7344c4f-ea97-579e-96a3-bbb681d3c7ef
DOI: 10.64898/2025.12.04.692101

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