Article

“Multiplex RT-PCR for SARS-CoV-2 variant surveillance in resource-limited settings: an in-house validation study in Cuba”

2026-06-25

Abstract excerpt

<h4>Background</h4> SARS-CoV-2 genomic surveillance is vital for public health, but whole-genome sequencing (WGS) remains costly and inaccessible in many resource-limited settings. We developed and validated a multiplex real-time RT-PCR assay for rapid, economical detection of key mutations associated with variants of interest (VOI) and concern (VOC). <h4>Methodology</h4> Two multiplex mixes (M1, M2) targeting e...

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Literature Corpus work: d3172b90-75ff-5b5b-bd52-b5fc07366fee
DOI: 10.64898/2026.06.22.26356299

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