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Identifying SARS-CoV-2 Variants of Concern through Saliva-Based RT-qPCR by Targeting Recurrent Mutation Sites.

Microbiology spectrum - 29 Jun 2022

Ham Rachel E, Smothers Austin R, Che Rui, Sell Keegan J, Peng Congyue Annie, Dean Delphine

Abstract excerpt

SARS-CoV-2 variants of concern (VOCs) continue to pose a public health threat which necessitates a real-time monitoring strategy to complement whole genome sequencing. Thus, we investigated the efficacy of competitive probe RT-qPCR assays for six mutation sites identified in SARS-CoV-2 VOCs and, after validating the assays with synthetic RNA, performed these assays on positive saliva samples. When compared with...

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Identifiers

PMID: 35546574
DOI: 10.1128/spectrum.00797-22
PMCID: PMC9241879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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