Article

Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase tagging

2020-05-17

Abstract excerpt

The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery but remains a major technical challenge. Here, we describe dynamic protein quantitation (DyProQ) which is effective across microscopy and multiwell platforms. Using collagen as a test protein, CRISPR-Cas9-mediated introduction of nluc (e...

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Literature Corpus work: cb6a6d07-a8a2-5458-a719-6e667f0b3271
DOI: 10.1101/2020.05.17.099119

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Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase taggingDOI 10.1101/2020.05.17.099119Read

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