Article

Collablots: Quantification of collagen VI levels and its structural disorganisation in cell cultures from patients with collagen VI-related dystrophies

2024-12-10

Abstract excerpt

<h4>Aims</h4> This study aims to develop a quantitative method for assessing collagen VI expression in cell cultures, which is crucial for the diagnosis and treatment of collagen VI-related dystrophies. <h4>Methods</h4> We developed a combined in-cell western (ICW) and on-cell western (OCW) assay, that we have called ‘collablot’ to quantify collagen VI and its organisation in the extracellular matrix of cell cul...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a23433f9-f8eb-577f-97c8-8aaf99e55c47
DOI: 10.1101/2024.12.06.627141

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Collablots: Quantification of collagen VI levels and its structural disorganisation in cell cultures from patients with collagen VI-related dystrophiesDOI 10.1101/2024.12.06.627141Read

Collagen VI is a conserved pro-fibrotic signal disrupting muscle regeneration across distinct human myopathiesSemantic proximity Read

Collagen VI disorders: Insights on form and function in the extracellular matrix and beyond.Semantic proximity Read

Collagen VI Muscle Disorders: Mutation Types, Pathogenic Mechanisms and Approaches to Therapy.Semantic proximity Read

Collagen VI in the Musculoskeletal System.Semantic proximity Read

Dynamic protein quantitation (DyProQ) of procollagen-I by CRISPR-Cas9 NanoLuciferase taggingSemantic proximity Read

Collagen type VI myopathies.Semantic proximity Read

Flow cytometry analysis: a quantitative method for collagen VI deficiency screening.Semantic proximity Read

Generation and Characterization of <i>Col6a1</i> knock-in mice: A Promising Pre-Clinical Model for Collagen VI-Related DystrophiesSemantic proximity Read

The expanded collagen VI family: new chains and new questionsSemantic proximity Read

Col6a1 knock-in mice provide a promising pre-clinical model for collagen VI-related dystrophies.Semantic proximity Read

Collagen XVIII in tissue homeostasis and dysregulation — Lessons learned from model organisms and human patientsSemantic proximity Read

Extracellular Matrix Disorganization and Sarcolemmal Alterations in COL6-Related Myopathy Patients with New Variants of COL6 Genes.Semantic proximity Read