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Semiquantitative Analyses of Naturally and Chemically Induced DNA Fragmentation in Human Sperm by Means of Angle-Modulated Two-Dimensional Single-Cell Pulsed-Field Gel Electrophoresis – Technical Instructions to Exclude False-Positive and -Negative

2025-07-21

Abstract excerpt

<h4>Background: </h4> To date, single-nuclear DNA damages in human sperm have been examined by means of the comet assay and the sperm chromatin structure assay. Revalidation using the comparative standards revealed their technical shortcomings. Methods We developed angle-modulated two dimensional single-cell pulsed-field gel electrophoresis (2D-SCPFGE) to detect the early symptoms of naturally occurring DNA fragme...

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Literature Corpus work: c6806128-95cc-576b-a841-8b997dd28bee
DOI: 10.20944/preprints202507.1612.v1

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Semiquantitative Analyses of Naturally and Chemically Induced DNA Fragmentation in Human Sperm by Means of Angle-Modulated Two-Dimensional Single-Cell Pulsed-Field Gel Electrophoresis – Technical Instructions to Exclude False-Positive and -NegativeDOI 10.20944/preprints202507.1612.v1Read

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