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Angle modulated two-dimensional single cell pulsed-field gel electrophoresis for detecting early symptoms of DNA fragmentation in human sperm nuclei

2023-10-04

Abstract excerpt

We here developed a novel angle-modulated two-dimensional single cell pulsed-field gel electrophoresis (2D-SCPFGE). Variations in current-application-time and rotation angle generated different alignments of DNA fibers and segments. After the first run, the specimen was turned by 150 degrees (2D-SCPFGE-0-150) to detect naturally occurring the earliest stage of DNA fragmentation or 75 degrees (2D-SCPFGE-0-75) to an...

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Literature Corpus work: c63e55a7-410a-53a4-99b9-7d5541308ac1
DOI: 10.21203/rs.3.rs-3382545/v1

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Angle modulated two-dimensional single cell pulsed-field gel electrophoresis for detecting early symptoms of DNA fragmentation in human sperm nucleiDOI 10.21203/rs.3.rs-3382545/v1Read

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