An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model

Abstract excerpt

Challenges related to high-quality RNA extraction from post-mortem tissue have limited RNA-sequencing (RNA-seq) application in certain skeletal muscle groups, including the intrinsic laryngeal muscles (ILMs). The present study identified critical factors contributing to substandard RNA extraction from the ILMs and established a suitable method that permitted high-throughput analysis. Here, standard techniques for...

Topics

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• Cancer-related molecular mechanisms research
• Cell Culture Techniques
• Disease Models, Animal
• Freezing
• Mice
• Molecular Biology Techniques and Applications
• Muscle, Skeletal
• Osteoarthritis Treatment and Mechanisms

Identifiers and source

Literature Corpus work

b632d6c4-18aa-59c5-b29e-cf88a6f7ec02

DOI

10.1101/2022.03.23.485541