Article

A novel method for the isolation of mycobacterial DNA

1996-01-01

Abstract excerpt

DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to −70 °C, then incubated at 65 °C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 × 109) or more cells) including Mycobacterium neoaurum M. fortuitum M. phlei an...

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Literature Corpus work: b50bcd02-2bc7-5716-a5a4-8bcc7751c5b8
DOI: 10.1016/0378-1097(95)00432-7

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