Article
High-Spatial-Resolution Multi-Omics Atlas Sequencing of Mouse Embryos via Deterministic Barcoding in Tissue
2019-10-01
Abstract excerpt
<h4>SUMMARY</h4> We present DBiT-seq –Deterministic Barcoding in Tissue for spatial omics sequencing – for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via NGS sequencing. Parallel microfluidic channels were used to deliver DNA barcodes to the surface of a tissue slide and crossflow of two sets of barcodes A1-50 and B1-50 followed by ligation in situ yielded a 2D mosaic of tissue pixels,...
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Identifiers and source
- Literature Corpus work
- b00cac5e-34ce-5f75-b7b9-c4e048ce6f19
- DOI
- 10.1101/788992