Article

PCR-based assays to detect and quantify Phomopsis sclerotioides in plants and soil

2010-02-01

Abstract excerpt

We developed polymerase chain reaction (PCR) assays to detect and quantify Phomopsis sclerotioides, the causal agent of black root rot of cucurbits. We used internal transcribed spacers 1 and 2 of the ribosomal DNA (rDNA) from representative isolates to search for target sequences. Primer pairs were selected after testing against 40 fungal isolates including 13 Ph. sclerotioides isolates, 9 Phomopsis isolates othe...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ac6255a0-750a-57eb-b555-3e453a1a6427
DOI: 10.1007/s10327-009-0209-6

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

PCR-based assays to detect and quantify Phomopsis sclerotioides in plants and soilDOI 10.1007/s10327-009-0209-6Read

Phomopsis Black Root Rot of Cucumber in Washington State Caused by Diaporthe sclerotioidesSemantic proximity Read

Morphological and molecular characterization of sclerotium rolfsii associated with fruit rot of Cucurbita maximaSemantic proximity Read

Development and evaluation of specific PCR and LAMP assays for the rapid detection of Phytophthora melonisSemantic proximity Read

Quantitative real-time PCR effectively detects and quantifies colonization of sclerotia of Sclerotinia sclerotiorum by Trichoderma sppSemantic proximity Read

Development of real-time PCR systems based on SYBR® Green I and TaqMan® technologies for specific quantitative detection of Phoma tracheiphila in infected CitrusSemantic proximity Read

Novel primers and PCR protocols for the specific detection and quantification of Sphingobium suberifaciens in situ.Semantic proximity Read

Multiplex qPCR for detection and quantification of Sphaerulina musiva in Populus stemsSemantic proximity Read

Quantitative PCR for detection and quantification of Phytophthora cactorum in the cultivation of strawberrySemantic proximity Read

Real-time PCR assays for the quantification of Phialophora gregata f. sp. sojae IGS genotypes A and B.Semantic proximity Read

Detection of cabbage yellows fungus Fusarium oxysporum f. sp. conglutinans in soil by PCR and real-time PCRSemantic proximity Read

Quantification of Cylindrocarpon destructans f. sp. panacis in soils by real-time PCRSemantic proximity Read

Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCRSemantic proximity Read