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Development of real-time PCR systems based on SYBR® Green I and TaqMan® technologies for specific quantitative detection of Phoma tracheiphila in infected Citrus

2008-04-01

Abstract excerpt

Real-time PCR assays based on SYBR® Green I and TaqMan® technologies were developed for in planta detection and quantification of Phoma tracheiphila, the mitosporic fungus causing 'mal secco' disease on citrus. Primers and a hybridization probe were designed on the basis of the internal transcribed spacer (ITS) region of the nuclear rRNA genes. The real-time PCR assays were compared with a classic isolation method...

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Literature Corpus work: 98d84a35-8d3a-5194-b4c5-631d270f94f0
DOI: 10.1007/s10658-007-9222-9

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