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Detection of cabbage yellows fungus Fusarium oxysporum f. sp. conglutinans in soil by PCR and real-time PCR

2016-09-01

Abstract excerpt

For the specific detection of Fusarium oxysporum f. sp. conglutinans (Foc), we designed a primer set for PCR and a primer–probe set for real-time PCR based on its endopolygalacturonase gene sequence. Using the primer set, we could distinguish Foc from other pathogenic forms and nonpathogenic isolates of F. oxysporum. Moreover, we detected the fungus by real-time PCR using DNA isolated from soil. Sensitivity of rea...

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Literature Corpus work: c52a9cb4-75b8-5476-a34a-44f121ac0fbb
DOI: 10.1007/s10327-016-0668-5

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