Article

Assessing Bias and Reproducibility of Viral Metagenomics Methods for the Combined Detection of Faecal RNA and DNA Viruses

2024-11-26

Abstract excerpt

Whole transcriptome amplification (WTA2) and sequence-independent single primer amplification (SISPA) are two widely used methods for combined metagenomic sequencing of RNA and DNA viruses. However, information on the reproducibility and bias of these methods on diverse viruses in faecal samples is currently lacking. A mock community (MC) of diverse viruses was developed and used to spike faecal samples at differe...

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Literature Corpus work: a94c3b5b-7a65-5f7e-97c5-092cdde4cf1a
DOI: 10.20944/preprints202411.2016.v1

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