Metatranscriptomics to characterize respiratory virome, microbiome, and host response directly from clinical samples

Abstract excerpt

We developed a metatranscriptomics method that can simultaneously capture the respiratory virome, microbiome, and host response directly from low biomass samples. Using nasal swab samples, we capture RNA virome with sufficient sequencing depth required to assemble complete genomes. We find a surprisingly high frequency of respiratory syncytial virus (RSV) and coronavirus (CoV) in healthy children, and a high...

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• Bacteriophages and microbial interactions
• Gut microbiota and health
• Respiratory viral infections research