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Comparison of PCR versus PCR-Free DNA Library Preparation for Characterising the Human Faecal Virome

18 Oct 2021

Abstract excerpt

The human intestinal microbiota is abundant in viruses, comprising mainly bacteriophages, occasionally outnumbering bacteria 10:1 and is termed the virome. Due to their high genetic diversity and the lack of suitable tools and reference databases, the virome remains poorly characterised and is often referred to as “viral dark matter”. However, the choice of sequencing platforms, read lengths and library...

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PMID: 34696523
DOI: 10.3390/v13102093

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