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Biases and complementarity in gut viromes obtained from bulk and virus-like particle-enriched metagenomic sequencing

2 Jul 2025

Abstract excerpt

Due to varying sequencing strategies, current gut virome findings show significant variability. Specifically, bulk- and virus-like particle (VLP)-enriched metagenomic sequencing (termed bulk and VLP, respectively) present unique advantages and limitations, affecting viral genome discovery, taxonomic annotation, and community structure analysis. A comprehensive comparison of these strategies is crucial for...

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PMID: 40600714
DOI: 10.1128/spectrum.00013-25

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