Article

smCounter2: an accurate low-frequency variant caller for targeted sequencing data with unique molecular identifiers

2018-03-14

Abstract excerpt

<h4>Motivation</h4> Low-frequency DNA mutations are often confounded with technical artifacts from sample preparation and sequencing. With unique molecular identifiers (UMIs), most of the sequencing errors can be corrected. However, errors before UMI tagging, such as DNA polymerase errors during end-repair and the first PCR cycle, cannot be corrected with single-strand UMIs and impose fundamental limits to UMI-ba...

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Literature Corpus work: a4b7f553-d8af-5710-94b7-3151105dbdfe
DOI: 10.1101/281659

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