Article

A novel PCR method directly quantifies sequence features that block primer extension

2018-05-17

Abstract excerpt

Many quantitative polymerase chain reaction (qPCR) assays indirectly quantify a target of interest as a drop in amplification signal relative to a larger total signal. The targeted sequence features include: DNA strand breaks introduced by enzymes that cleave at specific sequences or modifications, or caused by ionizing radiation or other DNA-damaging agents; DNA damage that does not break the DNA but blocks the D...

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Literature Corpus work: a3de4e88-5cef-5b92-bf53-f2a03e782de7
DOI: 10.1101/325308

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