Article

Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation

2020-08-04

Abstract excerpt

Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 953a5d3c-0b6c-5b2a-be60-07d869895360
DOI: 10.1101/2020.08.01.20166173

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparationDOI 10.1101/2020.08.01.20166173Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCRSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

THE USE OF DENATURING SOLUTION AS COLLECTION AND TRANSPORT MEDIA TO IMPROVE SARS-COV-2 RNA DETECTION AND REDUCE INFECTION OF LABORATORY PERSONNELSemantic proximity Read

SARS-CoV-2 detection by direct rRT-PCR without RNA extractionSemantic proximity Read

Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approachSemantic proximity Read

Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?Semantic proximity Read

Comparative effects of viral transport medium heat inactivation upon downstream SARS-CoV-2 detection in patient samplesSemantic proximity Read

Classic RNA extraction is efficient for subsequent detection of SARS-CoV-2 by RT-PCR in nasopharyngeal swab samplesSemantic proximity Read

Comparison of RNA extraction methods for the detection of SARS-CoV-2 by RT-PCRSemantic proximity Read

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detectionSemantic proximity Read

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detectionSemantic proximity Read