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SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

7 May 2020

Abstract excerpt

Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and...

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PMID: 32416598
DOI: 10.1016/j.jcv.2020.104423

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