Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

3 Feb 2021

Abstract excerpt

Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited new waves of infections in many places around the world. Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitated by simple, rapid, and inexpensive testing methods. This study evaluates several protocols for RNA extraction and RT-qPCR that are simpler and less expensive than...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Advanced biosensing and bioanalysis techniques
Share to Biosensors and Analytical Detection
Share to SARS-CoV-2 detection and testing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 33534838
DOI: 10.1371/journal.pone.0246647

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detectionPMID 33534838Read ↗

Ultrafast RNA extraction-free SARS-CoV-2 detection by direct RT-PCR using a rapid thermal cycling approachSemantic proximity Read ↗

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction stepSemantic proximity Read ↗

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCRSemantic proximity Read ↗

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read ↗

Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extractionSemantic proximity Read ↗

Validation of a single-step, single-tube reverse transcription-loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNASemantic proximity Read ↗

An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samplesSemantic proximity Read ↗

Comparative Analysis of Four Commercial RT-PCR Diagnostic Assay for Detection of Covid-19Semantic proximity Read ↗

An Open One-Step RT-qPCR for SARS-CoV-2 detectionSemantic proximity Read ↗

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read ↗

Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabsSemantic proximity Read ↗

An Open One-Step RT-qPCR for SARS-CoV-2 detectionSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.