Article

Mapping Protein Numbers in Living Cells

2021-07-03

Abstract excerpt

We introduce a new, robust method to map the numbers of proteins in living cells. The method can be applied to laser scanning, spinning disk, and lattice light-sheet microscopes in a robust, reproducible, and scalable fashion. The method uses calibrated EGFP solutions that are imaged with the appropriate microscope modality to create a calibration curve that is then applied to convert the fluorescence intensities...

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Identifiers and source

Literature Corpus work: 8dd0468f-f826-5797-be50-2a4e74d00c28
DOI: 10.1101/2021.07.02.450806

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