Article

RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding

2022-07-02

Abstract excerpt

<h4>ABSTRACT</h4> Progress in sample preparation for scRNA-seq is reported based on RevGel™-seq, a reversible-hydrogel technology optimized for samples of fresh cells. Complexes of one cell paired with one barcoded bead are stabilized by a chemical linker and dispersed in a hydrogel in the liquid state. Upon gelation on ice the complexes are immobilized and physically separated without requiring nanowells or drop...

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Literature Corpus work: 89762030-ecfa-566f-95e3-aa44a5e19fcd
DOI: 10.1101/2022.07.01.498266

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