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Droplet-based single-cell RNA sequencing: decoding cellular heterogeneity for breakthroughs in cancer, reproduction, and beyond

14 Oct 2025

Abstract excerpt

Droplet-based single-cell RNA sequencing (scRNA-seq) has redefined biological research by resolving cellular heterogeneity with an unprecedented precision. This review synthesizes the technological evolution of the 10× Genomics Chromium platform, its Gel Bead-in-Emulsion (GEM)s technology, and end-to-end workflows, while critically addressing persistent challenges, such as cell capture variability (30-75%...

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PMID: 41088302
DOI: 10.1186/s12967-025-06996-0

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