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A one step nested PCR method for detection of Peronospora sparsa, the downy mildew pathogen, in boysenberry (Rubus ursinus)

2021-08-01

Abstract excerpt

Downy mildew is a major disease of boysenberries in New Zealand, caused by Peronospora sparsa. Most boysenberry plant material, including tissue culture propagated plants are systemically infected and this pathogen also presents as a latent infection. The current nested PCR method to detect latent infection of P. sparsa in asymptomatic boysenberry plants is time consuming as it employs two separate PCR, with poten...

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Literature Corpus work
6e72ac5a-315d-5430-8349-5d2d00ef5c4e
DOI
10.1007/s10658-021-02283-y
Open publication

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A one step nested PCR method for detection of Peronospora sparsa, the downy mildew pathogen, in boysenberry (Rubus ursinus)DOI 10.1007/s10658-021-02283-y
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