Article

Specific detection of Phytophthora cactorum in diseased strawberry plants using nested polymerase chain reaction

2010-02-01

Abstract excerpt

Validated protocols for DNA purification and PCR amplification are reported for detection of Phytophthora cactorum in diseased strawberry plants. To remove PCR inhibitors, necrotic strawberry tissues were soaked in 5% alconox solution for >12 h before DNA extraction, and the extracted genomic DNA was embedded in an agarose gel chamber and subjected to electrophoresis. The purified DNA was amplified reliably by PCR...

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Literature Corpus work: ed6c4bf6-6bf0-591c-aaa0-1c4e443f0606
DOI: 10.1111/j.1365-3059.2009.02147.x

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