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A Quantitative Real-Time PCR Method to Detect the Quinoa Downy Mildew Pathogen, Peronospora variabilis.

Plant disease - 1 Sept 2024

Testen Anna Louise, Puri Purnima, Shaw R Scott, Domsic Evan Christopher, Griffin-LaHue Deirdre, Murphy Kevin Matthew, Mattupalli Chakradhar

Abstract excerpt

Quinoa downy mildew, caused by Peronospora variabilis, is the most devastating disease of quinoa globally. Rapid, sensitive diagnostic methods are needed to detect and quantify this pathogen in seeds and plant tissue. A hydrolysis probe-based quantitative real-time PCR (qPCR) assay including a competitive internal control was developed for P. variabilis detection. This assay could detect as low as 20 ag of DNA or...

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PMID: 38764340
DOI: 10.1094/PDIS-11-23-2308-RE

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