Article

Optimisation of single-nuclei isolation and RNA sequencing of parasitic nematodes

2024-11-13

Abstract excerpt

Single-cell/nuclei transcriptomics has revolutionised our understanding of cell, tissue, and organismal biology during health and disease of humans and model organisms. However, for non-model species, reassessment and refinement of conventional methods to address novel technical and biological challenges are needed. Here, we evaluate three established nuclei isolation protocols and, building on experiences, optimi...

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Literature Corpus work: 6afdf0b6-f139-5690-804e-026a60228965
DOI: 10.1101/2024.11.13.623282

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