Article
Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity in the genus Schistocerca.
STAR protocols - 19 Jun 2026
Techer Maéva A, Peralta Santana Vivian A, Woo Brandon, Marquess Richelle, Brennan Christopher, Mechti Audélia M C, Linde Jackson B, Behmer Spencer T, Sword Gregory A, Song Hojun
Abstract excerpt
Here, we present a protocol for generating gregarious and solitarious density-dependent phenotypes in multiple Schistocerca locust and grasshopper species under controlled environmental conditions. We describe cage setup, feeding, animal handling, and sterile dissection workflows to isolate nervous, chemosensory, gut, fat body, and female reproductive tissues from nymphs and adults. This protocol emphasizes rapid...
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