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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

7 Apr 2021

Abstract excerpt

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide...

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PMID: 33827654
DOI: 10.1186/s13059-021-02302-5

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