Article

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

2021-03-29

Abstract excerpt

Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of CRISPR sgRNA libraries into cell lines from several mosquito species and perform pooled CRISPR screens in an Anopheles cell line. To implement this method, we e...

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Literature Corpus work: 6a5affeb-42ba-54ed-b231-d6eb2906d9ec
DOI: 10.1101/2021.03.29.437496

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