Back to search

Article

Engineered Promoter System Enables High-efficiency Transgenic CRISPR Editing in Malaria Transmitting Mosquito <i>Anopheles sinensis</i>

2025-09-30

Abstract excerpt

The CRISPR/Cas9 system deployed through crosses of transgenic lines expressing Cas9 and gRNA facilitates efficient mutagenesis. However, its application in non-model insects remains limited, primarily due to a lack of well-characterized promoters capable of driving robust and stable expression of Cas9 and gRNA . In the malaria mosquito Anopheles sinensis , we evaluated several ovary-biased promoters— Asvasa2...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
0f28de43-637d-5d60-aab6-a631f2a2ed96
DOI
10.1101/2025.09.29.679135
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Engineered Promoter System Enables High-efficiency Transgenic CRISPR Editing in Malaria Transmitting Mosquito <i>Anopheles sinensis</i>DOI 10.1101/2025.09.29.679135
Highly efficient site-specific mutagenesis in Malaria mosquitoes using CRISPRSemantic proximity
Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPRSemantic proximity
High-efficiency gene editing in <i>Anopheles sinensis</i> using ReMOT controlSemantic proximity
Assessment of drive efficiency and resistance allele formation of a homing gene drive in the mosquito <i>Aedes aegypti</i>Semantic proximity
Cas9-Mediated Gene-Editing in the Malaria Mosquito Anopheles stephensi by ReMOT ControlSemantic proximity
CRISPR-mediated knock-in of transgenes into the malaria vector <i>Anopheles funestus</i>Semantic proximity
Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector, <i>Aedes aegypti</i>Semantic proximity
Gene drive-based population suppression in the malaria vector <i>Anopheles stephensi</i>Semantic proximity
Optimized CRISPR tools and site-directed transgenesis in <i>Culex quinquefasciatus</i> mosquitoes for gene drive developmentSemantic proximity
Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegyptiSemantic proximity
An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in <i>Drosophila</i>Semantic proximity
Methods for the generation of heritable germline mutations in the disease vector <i>Culex quinquefasciatus</i> using CRISPR/Cas9Semantic proximity
Select a neighboring publication to make it the new centre.